Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
نویسندگان
چکیده
منابع مشابه
Horizontal slice preparation of the retina.
Traditionally the vertical slice and the whole-mount preparation of the retina have been used to study the function of retinal circuits. However, many of retinal neurons, such as amacrine cells, expand their dendrites horizontally, so that the morphology of the cells is supposed to be severely damaged in the vertical slices. In the whole-mount preparation, especially for patch-clamp recordings,...
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Acknowledgements: We thank Susanne Wallenstein for excellent technical assistance, Ulrike Janssen-Bienhold for assistance with the PCR, and Jennifer Shelley for reading and improving the manuscript. 517). 2 GABA-induced currents have been characterized in isolated horizontal cells from lower vertebrates, but not in mammalian horizontal cells. Therefore, horizontal cells were isolated after enzy...
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Electrophysiological properties of a new isolated rat retina preparation
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GABA-induced currents have been characterized in isolated horizontal cells from lower vertebrates but not in mammalian horizontal cells. Therefore horizontal cells were isolated after enzymatical and mechanical dissociation of the adult mouse retina and visually identified. We recorded from horizontal cell bodies using the whole cell and outside-out configuration of the patch-clamp technique. E...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2017
ISSN: 1940-087X
DOI: 10.3791/55173